Idiomarina sp. Isolates from Cold and Temperate Environments as Biosurfactant Producers

Background: The cold-adapted Idiomarina sp. 185 from Antarctic shoreline sediment and the mesophilic A19 brackish Lake Faro (Italy) were screened for their efficiency in biosurfactant production by a temperature-mediated approach, when grown rich culture medium mineral supplemented with biphenyl. Methods: oxidation of polychlorobiphenyls standard screening tests performed, i.e., E24 index detection, surface tension measurement, blood agar plate C-TAB plate. Results: During incubation medium, strain produced an excellent stable emulsion, recording 73.5%. growth isolates showed good at least one performed condition showing species-specific differences related to optimum temperature. In presence biphenyl, both created emulsions (E24 ≈ 47.5 35%, respectively), as well reductions 30.05 35.5 mN/m, respectively. Further between observed phenotypic characterization. genome mining approach on available deposited sequences closest relatives offered further insights about genes biphenyl degradation, especially microorganisms derived different extreme environments. Conclusions: Our results allowed interesting comparison which underlined metabolic patterns kinetics BS production, probably due origins strains.